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anti mouse il 4 mab  (Bio X Cell)


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    Structured Review

    Bio X Cell anti mouse il 4 mab
    Anti Mouse Il 4 Mab, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+il+4+mab/InVivoMAb+anti-mouse+IFN%CE%B3/pm41086975-68-52-56
    Average 95 stars, based on 112 article reviews
    anti mouse il 4 mab - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression
    Article Snippet: .. Cells collected from mice were cultured in medium containing 5 mg/mL anti-mouse CD3 mAb (17A2, BioXcell), 1 mg/mL anti-mouse CD28 mAb (37.51.1, eBioscience), 50 mM2-mercaptoethanol (S6250, Sigma), 5 ng/mL human TGF-b (R&D), 20 ng/mL mouse IL-6 (Peprotech), 5 mg/mL anti-mouse IFN-g mAb (XMG1.2, BioXcell), and 5 mg/mL anti-mouse IL-4 mAb (11B11, BioXcell) for 3 days. .. Cells collected from humans were cultured in medium containing 5 mg/mL anti-human CD3 mAb (OKT3, eBioscience), 1 mg/mL soluble anti-human CD28mAb (CD28.2, eBioscience), 50 mM2-mercaptoethanol (Sigma), 2.5 ng/mL human TGF-b (R&D), 20 ng/mL human IL-6 (R&D), 10 ng/mL human IL-23 (R&D), 10 ng/mL human IL-1b (R&D), 5 mg/mL anti-human IFN-g mAb (285-IF-100, R&D), and 5 mg/mL anti-mouse IL-4 mAb (MP4-25D2, BioXcell) for 6–7 days.

    Article Title: Myeloid-derived suppressor cells shift Th17/Treg ratio and promote systemic lupus erythematosus progression through arginase-1/miR-322-5p/TGF-β pathway
    Article Snippet: .. Anti-mouse CD3 mAb, 5μg/ml (17A2, BioXcell); anti-mouse CD28 mAb, 1μg/ml (37.51.1, eBioscience); 2-mercaptoethanol, 50 μM (S6250, Sigma); human TGF-β, 2.5 ng/ml (R&D); mouse IL-6, 20 ng/ml (Peprotech); anti-mouse IFN-γ mAb, 5 μg/ml (XMG1.2, BioXcell); and anti-mouse IL-4 mAb, 5 μg/ml (11B11, BioXcell) were used, and cultured for 3 days. .. In some experiments, MDSCs (at a 1:1 ratio to the naı̈ve CD4+ T cells), N-hydroxy-nor-L-arginine (nor-NOHA, an Arg-1 inhibitor, at 300μM, Sigma) was added to determine the role of MDSCs and Arg-1 in Th17 cells differentiation.

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression
    Article Snippet: Cells collected from mice were cultured in medium containing 5 mg/mL anti-mouse CD3 mAb (17A2, BioXcell), 1 mg/mL anti-mouse CD28 mAb (37.51.1, eBioscience), 50 mM2-mercaptoethanol (S6250, Sigma), 5 ng/mL human TGF-b (R&D), 20 ng/mL mouse IL-6 (Peprotech), 5 mg/mL anti-mouse IFN-g mAb (XMG1.2, BioXcell), and 5 mg/mL anti-mouse IL-4 mAb (11B11, BioXcell) for 3 days. .. Cells collected from humans were cultured in medium containing 5 mg/mL anti-human CD3 mAb (OKT3, eBioscience), 1 mg/mL soluble anti-human CD28mAb (CD28.2, eBioscience), 50 mM2-mercaptoethanol (Sigma), 2.5 ng/mL human TGF-b (R&D), 20 ng/mL human IL-6 (R&D), 10 ng/mL human IL-23 (R&D), 10 ng/mL human IL-1b (R&D), 5 mg/mL anti-human IFN-g mAb (285-IF-100, R&D), and 5 mg/mL anti-mouse IL-4 mAb (MP4-25D2, BioXcell) for 6–7 days. .. To determine the role of MDSCs and polyamines in Th17 cell differentiation, MDSCs (at a 1:1 ratio to naive CD4+ T cells) were added in the presence or absence of ornithine (ORN, 100 nM, Sigma), difluoromethylornithine (DFMO, 20 nM, Tocris), putrescine (PUT, 2.5 mM, Sigma), spermidine (SPD, 2.5 mM, Sigma), or spermine (SPM, 2.5 mM, Sigma).

    Article Title: ALKBH3-AS1 drives SMAD3 stabilization via YTHDF2: Uncovering a pathogenic pathway for Th17 dysregulation in SLE.
    Article Snippet: .. Cells purified from mice were cultured in medium containing 5 μg/mL anti-mouse CD3 mAb (BE0001–1, BioXcell), 50 mM 2- mercaptoethanol (S6250, Sigma), 1 μg/mL anti-mouse CD28mAb (102102, BioLegend), 5 μg/mL anti-mouse IFN-g mAb (BE0045, BioXcell), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 20 ng/mL mouse IL-6 (216–16, PeproTech), and 5 μg/ mL anti-mouse IL-4 mAb (BE0055, BioXcell) for 3 days. .. Cells collected from humans were cultured in medium containing 50 mM 2-mercaptoethanol (Sigma), 5 μg/mL anti-human CD3 mAb (317349, BioLegend), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 1 μg/mL soluble anti-human CD28 mAb (BE0291, BioXcell), 10 ng/mL human IL-23 (1290-IL-10, R&D Systems), 5 μg/mL anti-human IFN-g mAb (285-IF-100, R&D Systems), 20 ng/mL human IL-6 (206-IL-200, R&D Systems), 10 ng/mL human IL1β (201-LB-025, R&D Systems), and 5 μg/mL anti-human IL-4 mAb (BE0240, BioXcell) for 6–7 days.

    Purification:

    Article Title: ALKBH3-AS1 drives SMAD3 stabilization via YTHDF2: Uncovering a pathogenic pathway for Th17 dysregulation in SLE.
    Article Snippet: .. Cells purified from mice were cultured in medium containing 5 μg/mL anti-mouse CD3 mAb (BE0001–1, BioXcell), 50 mM 2- mercaptoethanol (S6250, Sigma), 1 μg/mL anti-mouse CD28mAb (102102, BioLegend), 5 μg/mL anti-mouse IFN-g mAb (BE0045, BioXcell), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 20 ng/mL mouse IL-6 (216–16, PeproTech), and 5 μg/ mL anti-mouse IL-4 mAb (BE0055, BioXcell) for 3 days. .. Cells collected from humans were cultured in medium containing 50 mM 2-mercaptoethanol (Sigma), 5 μg/mL anti-human CD3 mAb (317349, BioLegend), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 1 μg/mL soluble anti-human CD28 mAb (BE0291, BioXcell), 10 ng/mL human IL-23 (1290-IL-10, R&D Systems), 5 μg/mL anti-human IFN-g mAb (285-IF-100, R&D Systems), 20 ng/mL human IL-6 (206-IL-200, R&D Systems), 10 ng/mL human IL1β (201-LB-025, R&D Systems), and 5 μg/mL anti-human IL-4 mAb (BE0240, BioXcell) for 6–7 days.



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